Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1253F

Mouse IgG (H+L chain) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Applications: ELISA, IF
• Recommended Dilution: Suitable for ELISA, Immunofluorescence Microscopy.
  Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  IF Microscopy: 1/1000-1/5000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Label: FITC
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/ml BSA (Ig and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons).
  Conjugation Chemistry: Isothiocyanate
  Absorption emission: 495 nm / 528 nm
  Molar radio: 3.1 moles FITC per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
• Conjugation: FITC
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.