Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1253F

Mouse IgG (H+L chain) rabbit polyclonal antibody, FITC


USD 205.00

3 Days*

Size
    • 2 mg

Product Images

Specifications

Product Data
Applications ELISA, IF
Recommended Dilution Suitable for ELISA, Immunofluorescence Microscopy.
Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1000-1/5000.
Host Rabbit
Clonality Polyclonal
Immunogen Mouse IgG whole molecule.
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Label: FITC
State: Lyophilized purified IgG fraction
Stabilizer: 10 mg/ml BSA (Ig and Protease free)
Preservative: 0.01% (w/v) Sodium Azide
Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons).
Conjugation Chemistry: Isothiocyanate
Absorption emission: 495 nm / 528 nm
Molar radio: 3.1 moles FITC per mole of Rabbit IgG
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Conjugation FITC
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.