Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1253T

Mouse IgG (H+L chain) rabbit polyclonal antibody, TRITC

Specifications

Product Data

• Applications: ELISA, IF
• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilution(s):
  FLISA : 1:10,000 - 1:50,000
  IF Microscopy : 1:1,000 - 1:5,000
  Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: TRITC
  State: Lyophilzed purified IgG fraction
  Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 3.0 moles TRITC per mole of Rabbit IgG
• Reconstitution Method: Reconstitute with 1.0 ml of deionized water or equivalent.
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below.
  Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.