Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1253TR

Mouse IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Specifications

Product Data

• Applications: IF
• Recommended Dilution: Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring high titer binding and lot-to-lot consistency.
  Recommended Dilutions:
  IF Microscopy : 1:500 - 1:2,500
  Other Dilution: User Optimized
  
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide as preservative.
  Label: Texas Red
  State: Lyophilized purified Ig fraction.
  Label: (TM) Sulfonyl Chloride (Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 5.7 moles Texas Red(TM) per mole of Rabbit IgG)
• Reconstitution Method: Restore with 1.0 ml of deionized water(or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: Texas Red
• Storage:
  Store vial at 4° C prior to reconstitution.
  Centrifuge product if not completely clear after reconstitution and standing at room temperature.
  This product is stable for several weeks at 4° C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20° C or below.
  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.