Goat IgG (H+L chain), adsorbed Donkey Polyclonal Antibody

CAT#: R1254HRP

Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP


USD 315.00

3 Days*

Size
    • 1 mg

Product Images

Specifications

Product Data
Recommended Dilution Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/130,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Host Donkey
Clonality Polyclonal
Immunogen Goat IgG whole molecule
Specificity Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Goat IgG and Goat Serum.
No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins
Formulation 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative
Label: HRP
State: Lyophilized purified Ig fraction
Label: Horseradish Peroxidase
Reconstitution Method Restore with 1.0 ml of deionized water or equivalent.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Conjugation HRP
Storage Store vial at 2-8°C prior to restoration.
Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.