Rabbit IgG (H+L chain) Sheep Polyclonal Antibody

CAT#: R1255AP

Rabbit IgG (H+L chain) sheep polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/15,000.
  Western Blot: 1/500-1/2,000.
  Immunohistochemistry: 1/200-1/1,000.
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0 with 0.01% Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
  Label: AP
  State: Liquid (sterile filtered) purified Ig fraction.
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 0.6 mg/ml (by UV absorbance at 280 nm)
• Purification: Prepared from monospecific antiserum by Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: AP
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  Freezing alkaline phosphatase conjugates will result in a substantial loss
  of enzymatic activity.
  Dilute only prior to immediate use.