Rabbit IgG (H+L chain) Sheep Polyclonal Antibody

CAT#: R1255B

Rabbit IgG (H+L chain) sheep polyclonal antibody, Biotin

Specifications

Product Data

• Recommended Dilution: Immunoblotting, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  ELISA: 1:300.000.
  Western blot and Immunohistochemistry: user optimized.
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% Sodium Azide as preservative
  Label: Biotin
  State: Lyophilized purified Ig fraction
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Sheep IgG molecule
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.