Rabbit IgG (H+L chain) Sheep Polyclonal Antibody

CAT#: R1255HRP

Rabbit IgG (H+L chain) sheep polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, immuno-peroxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/10,000-1/50,000.
  Western Blot: 1/1,000-1/5,000.
  Immunohistochemistry: 1/500-1/2,500.
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Label: Horseradish Peroxidase
  Molar radio: F/P (OD403/OD280) = 0.471
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.2 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Rabbit IgG coupled to agarose.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. Restore with deioinzed water (see 'Format'). Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing.