Rabbit IgG (H+L chain) Sheep Polyclonal Antibody

CAT#: R1255TR

Rabbit IgG (H+L chain) sheep polyclonal antibody, Texas Red

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended dilutions:
  Flow cytometry 1:2,000 - 1:10,000
  IF microscopy 1:500 - 1:2,500
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, and 0.01% (w/v) Sodium Azide
  Label: Texas Red
  State: Lyophilized purified IgG fraction
  Label: (TM) Sulfonyl Chloride (Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 2.2 moles Texas Red per mole of Sheep IgG
• Reconstitution Method: Restore with 2.0 ml of deionized water or equivalent.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
• Conjugation: Texas Red
• Storage: Store vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature.
  This product is stable for four weeks at 2-8°C as an undiluted liquid.
  For extended storage add glycerol to 50% and then aliquot contents and freeze at
  -20°C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.