Mouse IgG (H+L chain) Sheep Polyclonal Antibody

CAT#: R1256B

Mouse IgG (H+L chain) sheep polyclonal antibody, Biotin

Specifications

Product Data

• Recommended Dilution: Suiatble for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/10,000-1/50,000.
  Western Blot: 1/1,000-1/5,000.
  Immunohistochemistry: 1/1,000-1/2,000.
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum.
  This product is suitable for the detection of all Monkey immuno-globulin classes, isotypes and chain combinations.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
  Label: Biotin
  State: Lyophilized purified Ig fraction
  Label: Conjugation chemistry: N-hydroxysuccinimide (NHS) ester. Label MW: 455
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water r equivalent; this antibody is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below.
  Centrifuge product if not completely clear after standing at room temperature.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.