Mouse IgG (H+L chain) Sheep Polyclonal Antibody
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Specifications
Product Data | |
Recommended Dilution | Suiatble for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/5,000. Immunohistochemistry: 1/1,000-1/2,000. |
Host | Sheep |
Clonality | Polyclonal |
Immunogen | Mouse IgG whole molecule. |
Specificity | This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum. This product is suitable for the detection of all Monkey immuno-globulin classes, isotypes and chain combinations. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative. Label: Biotin State: Lyophilized purified Ig fraction Label: Conjugation chemistry: N-hydroxysuccinimide (NHS) ester. Label MW: 455 |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity chromatography. |
Conjugation | Biotin |
Storage | Store vial at 2-8°C prior to restoration. Restore with deionized water r equivalent; this antibody is stable for one month at 2-8°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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