Mouse IgG (H+L chain) Sheep Polyclonal Antibody

CAT#: R1256HRP

Mouse IgG (H+L chain) sheep polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/10,000-1/200,000
  Western blot: 1/1,000-1/5,000.
  Immunohistochemistry: 1/500-1/2,500.
• Reactivities: Mouse
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Mouse IgG and Mouse Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
  Label: HRP
  State: Lyophilized purified Ig fraction
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.