Mouse IgG (H+L chain) Sheep Polyclonal Antibody

CAT#: R1256P

Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, ID, IHC, IP, WB
• Recommended Dilution: Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/100,000.
  Western blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Reactivities: Mouse
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Mouse IgG and Mouse Serum.
  Anti-Mouse IgG whole molecule antibody generated in Sheep detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  State: Aff - Purified
  State: Liquid (sterile filtered) purified IgG fraction
  Stabilizer: None
  Preservative: 0.01% (w/v) Sodium Azide
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.