Chicken IgG / Chicken IgY (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1300B

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/500,000.
  Western blot: 1/2,000-1/20,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Chicken IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against Chicken immunoglobulins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Rabbit IgG molecule.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.5 mg/ml (by UV absorbance at 280 nm, before lyophil.)
• Purification: Immunoaffinity Chromatography.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.