Chicken IgG / Chicken IgY (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1300F

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Applications: FC, IF
• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Assay Dilutions:
  FLISA: 1/10,000-1/50,000.
  IF Microscopy: 1/1,000-5,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Chicken IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Chicken IgG and Chicken Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
  Label: FITC
  State: Lyophilized purified Ig fraction
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 3.9 moles FITC per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.