Chicken IgG / Chicken IgY (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1300HRP

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  Western blot: 1/1,000-1/10,000.
  ELISA: 1/10,000-1/50,000.
  Immunohistochemistry: 1/500-1/2500.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Chicken IgY/IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG. Some light chain cross reactivity may be observed against Chicken immunoglobulins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (Ig and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: HRP
• Storage: Store vial at 4°C prior to restoration.
  Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing.
  Dilute only prior to immediate use.