Chicken IgG / Chicken IgY (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1300T

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, TRITC

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Chicken IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Chicken IgG, and Chicken Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) Ig and Protease free as stabilizer and 0.01% (w/v) sodium azide as preservative
  Label: TRITC
  State: Lyophilized IgG fraction
  Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 2.9 moles TRITC per mole of rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 1.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2-8° C as an undiluted liquid.
  For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.