Chicken IgG / Chicken IgY (F(ab)2 specific) Rabbit Polyclonal Antibody

CAT#: R1302AP

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Chicken IgG F(ab')2 fragment.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab')2 and Chicken Serum.
  No reaction was observed against Chicken IgG F(c).
• Formulation: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 1 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) and 0.01% (w/v) and 0.01% Sodium Azide as preservative.
  Label: AP
  State: Liquid (sterile filtered) purified Ig fraction.
  Label: Alkaline Phosphatase (Calf Intestine, Molecular Weight 140,000 daltons)
• Concentration: 0.6 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  Freezing alkaline phosphatase conjugates will result in a substantial loss
  of enzymatic activity.
  Dilute only prior to immediate use.