Chicken IgG / Chicken IgY (H+L chain), adsorbed Goat Polyclonal Antibody

CAT#: R1303F

Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Flow cytometry: 1:2,000 - 1:10,000.
  IF Microscopy: 1:500 - 1:2,500.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Chicken IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Chicken IgG and Chicken Serum.
  No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.
• Formulation: PBS, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA; IgG and Protease free) as stabilizer and 0.01% sodium azide as a preservative
  Label: FITC
  State: Lyophilized purified Ig fraction
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 2.6 moles FITC per mole of Goat IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (OD280)
• Purification: Immunoaffinity chromatography
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.