Chicken IgG / Chicken IgY (H+L chain), adsorbed Goat Polyclonal Antibody

CAT#: R1303T

Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, TRITC

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Chicken IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum.
  No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as a stabilizer and 0.01% (w/v) Sodium Azide as preservative
  Label: TRITC
  State: Lyophilized Ig fraction
  Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 3.5 moles TRITC per mole of Goat IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: TRITC
• Storage: Store vial at 4°C prior to restoration. Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid.
  For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.