Bovine IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1304B

Bovine IgG (H+L chain) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Immunoblotting (1/2,000-1/10,000).
  ELISA (1/20,000-1/100,000).
  Immunohistochemistry (1/1,000-1/5,000).
  Immunomicroscopy.
  as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/20,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Bovine IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Bovine IgG and Bovine Serum.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Goat IgG molecule.
• Reconstitution Method: Restore with deionized water (or equivalent)
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.