Bovine IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1304F

Bovine IgG (H+L chain) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Applications: IF
• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Reactivities: Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Bovine IgG, whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Bovine IgG, Bovine IgG and Bovine Serum.
• Formulation: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4, with 0.01% (w/v) Thimerisol as preservative and 10 mg/ml Polyethylene Glycol (PEG mw 8,000) as stabilizer
  Label: FITC
  State: Lyophilized purified IgG fraction
  Label: Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) which has identical fluorescence properties as
  Absorption emission: 495 nm / 528 nm
  Molar radio: 4.2 moles DTAF per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.