Bovine IgG (Fc specific) Rabbit Polyclonal Antibody

CAT#: R1305B

Bovine IgG (Fc specific) rabbit polyclonal antibody, Biotin


USD 300.00

3 Days*

Size
    • 1.5 mg

Product Images

Specifications

Product Data
Applications ELISA, IF, IHC, WB
Recommended Dilution Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Note: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/15,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Reactivities Bovine
Host Rabbit
Clonality Polyclonal
Immunogen Bovine IgG F(c) fragment.
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum.
No reaction was observed against Bovine IgG F(ab')2.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
Label: Biotin
State: Lyophilized purified Ig fraction.
Molar radio: 10-20 BAC molecules per Rabbit IgG molecule.
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Concentration 1.5 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography.
Conjugation Biotin
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.