Bovine IgG (F(ab)2 specific) Rabbit Polyclonal Antibody

CAT#: R1306T

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, TRITC


USD 250.00

3 Days*

Size
    • 2 mg

Product Images

Specifications

Product Data
Applications IF
Recommended Dilution Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Host Rabbit
Clonality Polyclonal
Immunogen Bovine IgG F(ab')2 fragment.
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(ab')2 and Bovine Serum.
No reaction was observed against Bovine IgG F(c).
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with Polyethylene Glycol (PEG-8000) as stabilizer and 0.01% (w/v) sodium azide as preservative.
Label: TRITC
State: Lyophilized purified Ig fraction.
Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons)
Absorption emission: 550 nm / 570 nm
Molar radio: 2.7 moles TRITC per mole of Rabbit IgG.
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography.
Conjugation TRITC
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.