Feline IgG F(ab')2 specific Rabbit Polyclonal Antibody

CAT#: R1309F

Feline IgG F(ab')2 specific rabbit polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Cat IgG F(ab')2 fragment.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Cat IgG, Cat IgG F(ab')2 and Cat Serum.
  No reaction was observed against Cat IgG F(c).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: FITC
  State: Lyophilized purified Ig fraction.
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 3.0 moles FITC per mole of Rabbit IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.