Canine IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1310AP

Canine IgG (H+L chain) rabbit polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
  A working dilution of 1:1,000 to 1:5,000 is suggested for this product.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Dog IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Dog IgG and Dog Serum.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, with 10 mg/ml BSA (IgG and Protease free as stabilizer) and 0.01% (w/v) sodium azide as preservative
  Label: AP
  State: Liquid (sterile filtered) purified Ig fraction.
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
• Conjugation: AP
• Storage: Store the antibody undiluted at 2-8°C . DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.