Canine IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1310HRP

Canine IgG (H+L chain) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This antibody has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:16,000 to 1:72,000 of the reconstitution concentration is suggested for this product.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Dog IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgG and Dog Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as a stabilizer and 0.01% (w/v) Gentamicin Sulfate as a preservative
  Label: HRP
  State: Lyophilized Ig fraction
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Dog IgG coupled to agarose beads
• Conjugation: HRP
• Storage: Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 4°C as an undiluted liquid.
  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
  Dilute only prior to immediate use.