Canine IgG (Fc specific) Rabbit Polyclonal Antibody

CAT#: R1311HRP

Canine IgG (Fc specific) rabbit polyclonal antibody, HRP


USD 315.00

3 Days*

Size
    • 1.5 mg

Product Images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Host Rabbit
Clonality Polyclonal
Immunogen Dog IgG F(c) fragment
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgG, Dog IgG F(c) and Dog Serum.
No reaction was observed against Dog IgG F(ab')2.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Gentamicin Sulfate as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer
Label: HRP
State: Lyophilized purified Ig fraction
Label: Horseradish Peroxidase
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Concentration 1.5 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography
Conjugation HRP
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.