Goat IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1317AP

Goat IgG (H+L chain) rabbit polyclonal antibody, AP

Product Images

Western Blot of Rabbit anti-Goat Antibody Alkaline Phosphatase Conjugated: Lane 1: Goat IgG, Load: 100 ng per lane Secondary antibody Alkaline Phosphatase Conjugated Rabbit Anti-Goat Antibody at 1/1000 for 60 min at RT Block: MB-070 30 min RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/2,000-1/13,000.
  Western blot: 1/500-1/2,500.
  Immunohistochemistry: 1/200-1/1,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG and Goat Serum.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
  Label: AP
  State: Liquid (sterile filtered) purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.09% (w/v) Sodium Azide
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: AP
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  Dilute only prior to immediate use.