Goat IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1317F

Goat IgG (H+L chain) rabbit polyclonal antibody, FITC

Product Images

Western Blot of Rabbit anti-Goat IgG (H&L) Antibody Fluorescein Conjugated: Lane 1: Goat IgG, Load: 50 ng per lane Secondary antibody: Goat IgG (H&L) Antibody Fluorescein Conjugated at 1/1,000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa

Specifications

Product Data

• Applications: ELISA, IF
• Recommended Dilution: Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  Flow Cytometry: 1/500-1/2,500.
  IF Microscopy: 1/1,000-1/5,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG whole molecule
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: FITC
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 4.2 moles FITC per mole of IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent.
  Centrifuge product if not completely clear after standing at room temperature.
  This product is stable for several weeks at 2-8° C as an undiluted liquid.
  Dilute only prior to immediate use.
  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing.