Goat IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1317HRP

Goat IgG (H+L chain) rabbit polyclonal antibody, HRP

Product Images

Western Blot of Rabbit anti-GOAT IgG (H&L) Antibody Peroxidase Conjugated: Lane 1: Goat IgG, Load: 100 ng per lane Secondary antibody GOAT IgG (H&L) Antibody Peroxidase Conjugated at 1/1,000 for 60 min at RT Block: MB-070 for 30 min at RT

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/50,000-1/100,000.
  Western Blot: 1/5,000-1/20,000.
  Immunohistochemistry: 1/1,000-1/5,000.
  Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG, and Goat serum.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.