Goat IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1317T

Goat IgG (H+L chain) rabbit polyclonal antibody, TRITC

Specifications

Product Data

• Applications: ELISA, IF
• Recommended Dilution: Suitable for Immunomicroscopy (1/1,000-1/5,000) and Flow Cytometry (1/500-1/2,500) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG, whole molecule
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Goat IgG coupled to agarose beads.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: TRITC
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Tetramethylrhodamine isothiocyanante , Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 1.9 moles Rhodamine per mole of IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/mL (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.