Goat IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

CAT#: R1318F

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  Flow Cytometry: 1/500-1/2,500.
  IF Microscopy: 1/1,000-1/5,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG whole molecule.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum.
  No reaction was observed against Human Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Label: FITC
  State: Lyophilized purified Ig fraction.
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 2.8 moles FITC per mole of Rabbit IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C, reconstitute and store at 2-8°C for one month or at -20°C for longer.
  For extended storage reconstitute product with 50% Glycerol instead of water and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  Avoid repeated freezing and thawing.