Goat IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

CAT#: R1318HRP

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Product Images

Western Blot of HRP conjugated anti-Goat IgG antibody R1318HRP showing detection of 50ng of Goat IgG (Lane 1) but not Human IgG (Lane 2). Samples were separated by 4-20% SDS-PAGE under reducing conditions and transferred to nitrocellulose membrane. The blot was blocked overnight at 4°C in 5% BSA in TBS. A 1/5,000 dilution of antibody in Blocking Buffer for Fluorescent Western Blotting was used to probe the membrane at RT for 1 h. The image was developed using Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate (p/n Femtomax-020) for one minute.

Specifications

Product Data

• Recommended Dilution: Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended dilutions
  ELISA: 1/10,000-1/50,000.
  Western blot: 1/1,000-1/5,000.
  Immunohistochemistry: 1/500-1/2,500.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum.
  No reaction was observed against Human Serum Proteins.
• Formulation: 0.02 MPotassium Phosphate, 0.15M Sodium Chloride, pH 7.2, with 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
  Label: HRP
  State: Lyophilized purified Ig fraction
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml deionized water or equivalent.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.