Goat IgG (Fc specific) Rabbit Polyclonal Antibody

CAT#: R1319AP

Goat IgG (Fc specific) rabbit polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:400 to 1:2,800 of the reconstitution concentration is suggested for this product.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG F(c) fragment.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum.
  No reaction was observed against Goat IgG F(ab').
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
  Label: AP
  State: Liquid (sterile filtered) purified Ig fraction
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 0.73 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  Dilute only prior to immediate use.