Goat IgG (Fc specific) Rabbit Polyclonal Antibody

CAT#: R1319HRP

Goat IgG (Fc specific) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Western Blot/Dot Blot: 1/1,000-1/10,000 or Dot blot.
  Immunohistochemistry: 1/500-1/2,500.
  ELISA: This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 is suggested for this product.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG F(c) fragment.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum.
  No reaction was observed against Goat IgG F(ab')2 .
• Formulation: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (Ig and Protease free) as stabilizer.
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Goat IgG coupled to agarose beads.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration.
  After reconstitution, this antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  Avoid repeated freezing and thawing.