Guinea pig IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1322F

Guinea pig IgG (H+L chain) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  Flow Cytometry: 1/500-1/2,500.
  IF Microscopy: 1/1,000-1/5,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Guinea Pig IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Guinea Pig IgG and Guinea Pig Serum.
• Formulation: 0.01M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% (w/v) Thimerisol as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
  Label: FITC
  State: Lyophilized purified Ig fraction.
  Label: Molecular Weight 390 daltons
  Absorption emission: 495 nm / 528 nm
  Molar radio: 2.0 moles DTAF per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.2 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.