Hamster IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1327B

Hamster IgG (H+L chain) rabbit polyclonal antibody, Biotin


USD 245.00

3 Days*

Size
    • 1.5 mg

Product Images

Specifications

Product Data
Recommended Dilution Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0µg of hamster IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/15,000 to 1/60,000 of the reconstitution concentration is suggested for this product.
Host Rabbit
Clonality Polyclonal
Immunogen Hamster IgG whole molecule
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Hamster IgG and Hamster Serum.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, and 0.01% (w/v) Sodium Azide
Label: Biotin
State: Lyophilized IgG fraction
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
Reconstitution Method Restore with 1.0 ml of deionized water or equivalent.
Concentration 1.5 mg/ml (OD 280 nm)
Purification Immunoaffinity chromatography using Hamster IgG coupled to agarose
Conjugation Biotin
Storage Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.