Hamster IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1327HRP

Hamster IgG (H+L chain) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA 1:16,000 - 1:64,000
  WESTERN BLOT 1:1,000 - 1:5,000
  IMMUNOHISTOCHEMISTRY 1:500 - 1:2,500
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Golden Syrian Hamster IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Hamster IgG and Hamster Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.5 mg/ml (by UV absorbance at 280 nm, before lyophil.)
• Purification: Immunoaffinity chromatography.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.