Golden Syrian Hamster IgG (F(ab)2 specific) Rabbit Polyclonal Antibody

CAT#: R1328AP

Golden Syrian Hamster IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  This product has been assayed against 1.0 ug of Hamster IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Hamster IgG F(ab')2 fragment
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Hamster IgG and Hamster Serum.
  No reaction was observed against Hamster IgG F(c).
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, and 0.01% (w/v) Sodium Azide
  Label: AP
  State: Liquid (sterile filtered)
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Hamster IgG coupled to agarose
• Conjugation: AP
• Storage: Store vial at 4°C before opening. DO NOT FREEZE. This product is stable at 4°C as an undiluted liquid. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  Dilute only prior to immediate use.