Equine IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1329TR

Equine IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Specifications

Product Data

• Recommended Dilution: This product is designed for Immunofluorescence Microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000
  Immunofluorescence: 1/1,000-1/5,000
  Flow Cytometry: 1/500-1/2,500
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Horse IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Horse IgG and Horse Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Texas Red
  State: Lyophilized purified Ig fraction.
  Label: (TM) Sulfonyl Chloride (TR; Molecular Weight 625 daltons)
  Absorption emission: 596 nm Emission Wavelength: 620 nm
  Molar radio: 2.4 moles Texas Red per mole of Rabbit IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: Texas Red
• Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.