Human IgG (H+L chain) Chicken Polyclonal Antibody
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Specifications
Product Data | |
Recommended Dilution | ELISA: 1/2,000 - 1/10,000. Western Blot: 1/500 - 1/2,500. Immunohistochemistry: 1/200 - 1/1,000. |
Host | Chicken |
Clonality | Polyclonal |
Immunogen | Human IgG whole molecule. |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Chicken Serum, Human IgG and Human Serum. |
Formulation | 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) and 0.01% (w/v) Sodium Azide as preservative Label: AP State: Liquid (sterile filtered) purified IgG fraction Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity chromatography |
Conjugation | AP |
Storage | Store the antibody at 2-8°C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use. |
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.