Human IgG (H+L chain) Chicken Polyclonal Antibody

CAT#: R1332TR

Human IgG (H+L chain) chicken polyclonal antibody, Texas Red

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Host: Chicken
• Clonality: Polyclonal
• Immunogen: Human IgG, whole molecule.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, Human IgG and Human Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
  Label: Texas Red
  State: Lyophilized purified Ig fraction.
  Label: (TM) Sulfonyl Chloride (Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 4.1 moles Texas Red(TM) per mole of Chicken Ig
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using human IgG coupled to agarose beads
• Conjugation: Texas Red
• Storage: Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.