Human IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

CAT#: R1334P

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/75,000-1/500,000.
  Western Blot: 1/10,000-1/50,000.
  Immunohistochemistry: 1/1,000-1/10,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum.
  No reaction was observed against Mouse Serum Proteins. Specificity was confirmed using ELISA at less than 0.9% cross reaction against Mouse IgG.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  State: Aff - Purified
  State: Liquid (sterile filtered) purified Ig fraction.
  Stabilizer: None
  Preservative: 0.01% (w/v) Sodium Azide
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.