Human IgG (H+L chain), adsorbed Goat Polyclonal Antibody

CAT#: R1335HRP

Human IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution:
  ELISA: 1/200,000.
  Western Blot: 1/1,000-1:5,000.
  Immunohistochemistry: 1/500-1/2,500
  Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Human IgG whole molecule.
• Specificity: Anti-Human IgG (H&L) Peroxidase generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum.
  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Note: Centrifuge HRP Conjugated Secondary Antibody if not completely clear after standing at room temperature.