Human IgG (gamma chain specific) Rabbit Polyclonal Antibody

CAT#: R1336P

Human IgG (gamma chain specific) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/20,000-1/100,000.
  Western Blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human IgG gamma chain heavy chain.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum.
  No reaction was observed against Human IgM or Human IgA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Human heavy or light chain isotypes.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 0.01% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) purified IgG fraction
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Storage: Store vial at 2-8°C prior to opening. This product is stable for several weeks at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
  For extended storage aliquot contents and freeze at -20° C or below.
  Avoid cycles of freezing and thawing.