Human IgG (gamma chain specific) Rabbit Polyclonal Antibody

CAT#: R1336T

Human IgG (gamma chain specific) rabbit polyclonal antibody, TRITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  IF Microscopy: 1/1,000-1/5,000.
  Flow Cytometry: 1/500-1/2,500.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human IgG heavy chain
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, and Human Serum.
  Less than 1% cross reactivity was observed by ELISA against other Human heavy and light chain proteins.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: TRITC
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 3.0 moles TRITC per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.