Human IgG (gamma chain specific) Rabbit Polyclonal Antibody

CAT#: R1336TR

Human IgG (gamma chain specific) rabbit polyclonal antibody, Texas Red

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  IF Microscopy: 1/1,000-1/5,000.
  Flow Cytometry: 1/500-1/2,500.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human IgG gamma heavy chain.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum.
  No reaction was observed against Human IgM or Human IgA.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Texas Red
  State: Lyophilized purified Ig fraction.
  Label: (R) Sulfonyl Chloride (TR; Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 2.5 moles Texas Red per mole of Rabbit IgG
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: Texas Red
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.