Human IgG (Fc specific) Rabbit Polyclonal Antibody

CAT#: R1337F

Human IgG (Fc specific) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: This Fluorescein Conjugated antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  IF MIscroscopy: 1/1000-1/5000.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Immunogen Human IgG F(c).
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum.
  No reaction was observed against Human IgG F(ab')2.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide as preservative.
  Label: FITC
  State: Lyophilized purified Ig fraction.
  Label: Fluorescein isothiocyanate ; Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 2.6 moles FITC per mole of Rabbit IgG.
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.