Human IgG (Fc specific) Rabbit Polyclonal Antibody

CAT#: R1337HRP

Human IgG (Fc specific) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/10,000-1/50,000.
  Western Blot: 1/1,000-1/5,000.
  Immunohistochemistry: 1/500-1/2,500
  Note:This antibody has been assayed against 1.0 ug of Human IgG F(c) in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human IgG F(c) fragment
• Specificity: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum.
  No reaction was observed against Human IgG F(ab')2.
• Formulation: 0.02 M Potassium Phosphate, 0.14 M Sodium Chloride, pH 7.4, containing 10 mg/ml BSA (IgG and Protease free) as a stabilizer and 0.01% (w/v) Gentamicin Sulfate as a preservative.
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.