Human IgG (F(ab)2 specific) Rabbit Polyclonal Antibody

CAT#: R1338F

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human IgG F(ab')2 fragment
• Specificity: This antibody detects Human IgG F(ab)2 Fragment.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum. No reaction was observed against Human IgG F(c).
• Formulation: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4; 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; 0.01% (w/v) Thimerisol
  Label: FITC
  State: Lyophilized Ig fraction
  Label: Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 2.8 moles DTAF per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
• Conjugation: FITC
• Storage: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.