Human IgG (F(ab)2 specific) Rabbit Polyclonal Antibody

CAT#: R1338HRP

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP


USD 300.00

3 Days*

Size
    • 2 mg

Product Images

Specifications

Product Data
Recommended Dilution Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. 
Recommended Dilutions: 
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/10,000.
Immunohistochemistry: 1/500-2,500.
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product.
Host Rabbit
Clonality Polyclonal
Immunogen Human IgG F(ab')2 fragment
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F(ab)2 and Human Serum. No reaction was observed against Human IgG F(c).
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Label: HRP
State: Lyophilized Ig fraction
Stabilizer: 10 mg/ml BSA (IgG and Protease free)
Preservative: 0.01% (w/v) Gentamicin Sulfate
Label: Horseradish Peroxidase
Reconstitution Method Restore with 1.0 ml of deionized water or equivalent.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography
Conjugation HRP
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.